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Eudicot sequences were mixed in different groups, with no evidences of species-specific proliferations.
To address the question of PCR recombination, 1 ng of DNA of three different molecularly cloned CTNNB1 sequences were mixed at an equimolar ratio and 3DPCR performed using the same 7 °C gradient.
Using a TDA (template-directed assembly) approach which organizes functional complexes of membrane-associated proteins, His-tagged DDR2 and/or Src (see Supplementary Figure S1C at http://www.biochemj.org/bj/454/bj4540501add.htm for sequences) were mixed at the indicated amounts with a 1 5000 ratio of TDA/enzyme.
In order to build a reference curve for measuring the levels of heteroplasmy in the biological samples, plasmids containing the common (C150) and the mutant (150T) sequences were mixed in different proportions (0% C with 100% T; 5% C with 95% T; 10% C with 90% T; 20% C with 80% T; 30% C with 70% T; 40% C with 60% T; 50% C with 50% T) and again subjected to PCR amplification.
Appropriately designed oligopeptides containing either RGD or PHSRN bioactive sequences were mixed and covalently bonded on CPTES-silanized surfaces.
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Other WD-40 sequences are mixed up.
The evidence for diversifying or positive selection on our putative toxin sequences was mixed.
Functional TF binding sites (TFBSs) can be identified in the genome by computational approaches or experimentally by Chromatin ImmunoPrecipitation and hybridization on a genomic microarray (ChIP on Chip) [ 2] or by high-throughput selection procedures (SELEX) in which pools of random DNA sequences are mixed with a TF and those that are preferentially bound are recovered and sequenced [ 3, 4].
Samples to be pooled for sequencing were mixed in equal quantities and concentrated using QIAquick PCR purification kit (Qiagen) before sequencing on the GS FLX Titanium sequencer (Agowa GmBH, Berlin, Germany).
The GB1 template was produced by an overlap extension PCR in which four overlapping primers covering the complete GB1 sequence were mixed.
Integration into the original target sequence DNA in the substrate DNA was significantly reduced when the substrate DNA including modified sequence was mixed.
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