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The rates of recombination between homologous and homeologous sequences were measured using specific engineered tools (Fig. 3a, Supplementary Fig. 2A,B).
The integration efficiencies of 52 mutated loxP sequences, including novel sequences, were measured using an in vitro evaluation system.
The binding affinities of the conjugates to their target sequences were measured by surface plasmon resonance (BIAcor) techniques.The peptide-oligonucleotide conjugates progressively entered cells over a period of hours and were detected in cytoplasmic vesicles and in the nucleus.
Regional fractions of repetitive sequences were measured and averaged by regional bin.
The expression levels of these sequences were measured in Escherichia coli and found to be highly correlated (R 2 = 0.87) with their estimated translational efficiencies.
Position-dependent compositions in the terminal sequences were measured in an 11 nucleotide sliding window, advancing by one nucleotide steps over the 600 nt region.
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In order to estimate PSNRpeak, PSNRpeak/PSNRfull ratios of 12 video sequences are measured under various target bitrates and at various values of slope α.
The relative cleavage rate of 3CLpro against these substrate sequences was measured (Figure 2, Table S1).
DNA distance among sequences was measured using dnadist of the PHYLIP package (Felsenstein 1989) version 3.6.
Therefore, the TpA/ApT ratio across the various Ceratocystis Fot5 sequences was measured.
GC content of the sequences was measured using Emboss GeeCee tool, while sequences were scanned for SSR markers using MISA [ 113].
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