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Clone sequences affiliating to the Chrysophyceae mainly clustered within the clade C2, which has been known so far for its presence mainly in cold climatic regions, whereas Kinetoplastea sequences were mainly related to the Neobodonid clade.
A large number of alkane MO sequences retrieved in this study showed the highest similarity with reference sequences belonging to Rhodococcus genus, suggesting a key role of this genus in TeCA/propane co-metabolism, while the remaining alkane MO sequences were mainly attributed to other Actinobacteria, to Bradhyrizobiaceae, and Cupriavidus genus.
Among Stramenopiles, BIOSOPE sequences were mainly distributed within Pelagophyceae, Chrysophyceae, and Dictyochophyceae (Figure 2).
We additionally clustered sequences by length to see if the sheer number of insertions and deletions in divergent sequences were mainly responsible for this effect.
As expected, these sequences were mainly associated to photosynthesis.
To exclude possible cross matching, the 3'-untranslated sequences were mainly used for the reverse primers.
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The fore-main MS sequences are mainly controlled by excavation unloading in the tunnel.
The terrestrial sequences are mainly Late Miocene in age (c. 12 5 Ma) and conform to five main types: aa lava-fed deltas, volcanic sheet-like sequences, a glaciolacustrine sequence, scoria cones and tuff cones.
As a consequence, MR with MRCP sequences is mainly performed to study the biliary and pancreatic ductal systems and anastomoses.
Clone library analysis (Maidak et al. 1999) based on 16S rRNA gene sequences was mainly carried out to determine the bacterial community compositions in these soil samples.
The remaining sequences are mainly from viral types with significant similarity to approximately 30 viral families.
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