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The ITE/ARIB Hi-Vision Test Sequences (ITE sequences) were made for HDTV assessments, and the period of each sequence is 15 s [26, 27].
Comparisons of the nucleotide sequences were made using BlastN [44].
Alignments of N- and C-terminal amino acid sequences were made separately using default parameters in ClustalW (MacVector™).
All sequences were made to be 147 bp in length, and the reverse compliments of these sequences were also analyzed.
Multiple sequence alignments of the deduced amino acid sequences were made using Multiple Alignment software (http://www.ebi.ac.uk/clustalw/index.html) [47].
Multiple sequence alignments of deduced amino acid sequences were made using the ClustalW multiple-alignment software (http://www.ebi.ac.uk/clustalw/index.html).html
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Comparison with already known sequences was made with the BLAST program (Basic Local Alignment Search Tool, blast.ncbi.nlm.nih.gov) from the National Center for Biotechnology Information.
A list of all possible extension sequences is made (one sequence per overhanging read).
Furthermore, the tardigrade analyzer, a sequence server to analyse individual tardigrade specific sequences, is made available.
Finally, an alignment of these sequences is made using the profile.
The alignment of rpoB and MLST gene sequences was made by use of ClustalW2 [ 16].
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