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The promoter sequences were isolated and tested in transgenic rice lines using a GUSplus reporter gene.
Drosophila alpha-spectrin cDNA sequences were isolated from a lambda gt11 expression library.
After 10 iterative selection cycles, 35 TA sequences were isolated.
Viral sequences were isolated from each subject and analyzed using phylogenetic methods.
However, in the other cases, several sequences were isolated, suggesting that deletions of these genes are relatively easy to rescue.
By using a degenerate primer pair [20], [21], 21 WRKY sequences were isolated from Cocos nucifera [22].
Cleaved sequences were isolated by denaturing PAGE and PCR-amplified with primers PM1 and PM2 (Figure 1 inset).
Transcribed Xist sequences were isolated from O. palustris and S. hispidus by a combination of PCR and RT-PCR.
In addition, partial sequences were isolated from Diacrisia sannio (DsFMO and DsPNO) and Estigmene acrea (EaFMO and EaPNO) and an additional sequence A. villica (AvPNO).
Methylated DNA sequences were isolated using the MethylMiner kit (Invitrogen).
3'-end retroelement sequences were isolated from pinot noir S15.
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