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Aligned sequences were inspected and manually adjusted when necessary.
Sequences were inspected, aligned, and edited using MacVector® 8.0.2 software.
Individual sequences were inspected, verified and assembled into contigs using Lasergene Seqman software (DNASTAR).
Resulting sequences were inspected and aligned using Geneious Pro 4.8.5 [30] to implement either ClustalW [31] or Muscle [32].
The candidate sequences were inspected individually, to select the sequences showing a large number of repeat units, as well as sufficient flanking region and base composition, suitable for primer design.
These Hong Kong HA and NA sequences were inspected to determine if there were any sequences from consecutive influenza seasons occurring on the same branch, indicating that viruses with the same or very similar HA and NA gene sequences were occurring in adjacent influenza seasons.
Similar(23)
The identified proteins are contrasted by computational analysis and their sequences are inspected to correct possible errors in function prediction.
The trimmed sequences were assembled using CAP3 [ 30] with 21-bp overlap and 85% identity; the quality of the assembled sequences was inspected visually using Sequencher 4.5 (Gene Codes) and Blast [ 49].
The peptides released into the incubation buffer (the "surfome" or surface proteome) are analysed by LC/MS/MS, the identified proteins are validated by computational analysis and their sequences are inspected to correct possible errors in function prediction.
The three-frame translations of each sequence were inspected for the presence of a signal peptide using the SignalP server [ 21].
In cases where sequences from two strains had signal peptides predicted but the orthologous sequence from the third did not, the start codon region of that sequence was inspected manually, based on the amino acid sequence alignment with its orthologs.
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