Exact(5)
The amiRNA sequences were incorporated in an Arabidopsis thaliana miR168a gene backbone built in a plant binary vector, [ 29, 30].
On the other hand, when identical predicted sequences were incorporated in miRNA scaffolds, two out of three sequences resulted in potent inhibition of luciferase reporters and endogenous mRNA.
Additionally, the same siRNA sequences were incorporated in artificial miRNA scaffolds and direct evaluation between the silencing efficacy and processing of pol II-expressed shRNA and miRNA was performed.
As our analysis continued and more genome sequences were incorporated in our studies we realized that most of these identified multimodular proteins actually contained multiple domains and had one overall function.
A possible explanation for this discrepancy is that the small RNA TaqMan assay detects only one variant of the guide strand and although identical predicted siRNA sequences were incorporated in the CMV-shRNA and CMV-miRNA scaffolds, they can still be processed differently.
Similar(55)
Where identical DNA sequences are incorporated in a single mRNA transcript, as shown in designs II and III of the XOR gate, the effect of 5′-UTR secondary structure preventing gene expression is significantly more pronounced.
The pri-miRNA-encoding sequences are incorporated in the 3′UTR of a Renilla luciferase gene and are concomitantly expressed with the EGFP gene from a common, constitutive CMV promoter.
RT sequences were incorporated into TyHRT elements by in vivo homologous recombination following transformation into Saccharomyces cervisiae.
In total, 19.45 Mb of previously unplaced genome sequences were incorporated into the new version of the genome assembly.
These new sequences were incorporated into our analysis (Fig. 3).
When a 'bait' sequence was incorporated in a donor plasmid, simultaneous cutting of the genomic DNA and the plasmid using CRISPR/Cas9 system enabled high efficiency site-specific transgenesis.
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