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The sequences were further clustered into 317 OTUs using a 97% similarity cut-off.
The debatable sequences were further discussed until a full consensus was reached.
All these lectin sequences were further classified into nine of the 12 plant lectin families (Table 1).
These sequences were further clustered to identify unique sequences.
The downstream and upstream regions of the known sequences were further obtained using genome walking-PCR.
The three sequences were further analysed using maximum likelihood (ML) inference.
These 12 candidate sequences were further examined by Northern blot to confirm their size and sequence.
The output predicted protein sequences were further blasted in order to have phylogenetic and function estimation.
PARP3-bound sequences were further examined for a potential enrichment in specific DNA sequences.
Sequences that with complete match to the genome sequences were further analyzed for differential expression.
The predicted protein sequences were further defined with Psort [60], TMHMM [61], SignalP [62] and InterProScan [63].
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