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We validated the genome assembly using 27 BAC sequences from CM334: 26 BAC sequences were fully covered by a single or multiple scaffolds and showed identities of greater than 99.9% (Supplementary Fig. 4 and Supplementary Table 5).
Twelve sequences were fully transcoded, where the total number of frames for every one of them was simulated.
Similar procedures were used to obtain partial cDNA sequences from the other equid species, using additional primer sets designed to overlap those of the original primer positions to ensure that the final sequences were fully correct for each species.
The results showed that 8 sequences were fully matched, while 7 sequences had one or two mismatched nucleotides (Table 3).
In the H-I loop (residues 172 182), all the 19 sequences were fully conserved at positions 172, 176 and 181 182.
Clones were initially sequenced with a 519 533 reverse primer and those that differed from known reference sequences were fully sequenced using multiple primers on each strand.
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In Uncharted, dialogue sequences are fully scripted and animations for each dialogue are hand crafted.
Two thirds of these sequences are fully identified; the oldest sequence with an unsatisfactory BLAST match has resided in INSD for a full 14 years.
Argonaute 2 (AGO2) is the catalytic engine of RNAi that allows cleavage of target RNAs when sequences are fully complementary.
Mj024A04 BAC clone randomly selected from BAC clones that possessed black tiger shrimp IAP sequences was fully sequenced and 27 genes were predicted in silico.
Indeed, whereas these sequences are fully divergent in Pim2 and Pim3, Disoprep analysis shows that the C-terminal sequence of Pim3 is also disordered.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com