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Transcripts containing HERV-W gag sequences were expressed in non-random patterns with extensive variations in the expression between both tissues, including different brain regions, and individuals.
In contrast, when the ≥1 kb random DNA (RD) sequences were expressed as part of the 3′-untranslated region (UTR) transgene silencing was not observed.
We first verified by RT-PCR that 8b and 9b sequences were expressed in the dentate gyrus and the CA regions of the hippocampus (Figure 4).
Interestingly, however, when the full-length wild-type Xenopus sequences were expressed, the receptor was mainly found at the plasma membrane and evenly distributed intracellularly with less than 5% of the cells showing any nuclear localization (Table 2, Fig. 4).
Transgenic flies carrying the TBPHF/L150 152 construct under UAS sequences were expressed in neurons using elav-GAL4 and it was found that TBPH without RNA binding activity was not able to rescue the TBPH loss of function phenotypes compared with endogenous TBPH, although the intracellular localization of these constructs was similar in both the cases (Figure S5A).
For yeast experiments, coding DNA sequences were expressed from inducible Gal1/10 promoters.
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It would be interesting to evaluate whether these intronless sequences are expressed or whether the loss of the intron completely abolished gene expression, rendering these genes as pseudogenes.
Such sequences are expressed by (4).
That the activity specific to sequences is expressed solely in prefrontal cortex is especially interesting when considered in light of current theories of prefrontal function.
Indeed, we showed that 8b and 9b sequences are expressed in both the dentate gyrus and CA regions of the hippocampus.
The remaining 57 sequences are expressed in the relevant parasite life-stages that persist in humans, namely, the immature schistosomulum form, either prepared in vitro or removed from a mammalian host (in vivo), adult (male and/or female) and egg.
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