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Nuclear sequences were examined using GeneJockey to find a restriction site that would distinguish them using Polymerase Chain Reaction-Restriction Fragment Length PCR RFLPhism (PCR RFLP).
Sequences were examined using Codon Code Aligner Software®.
Sequences were examined using the Chromas 2.01 software (Technelysium Pty Ltd).
Aligned sequences were examined using PAUP [ 57] by testing models supplied by Modeltest [ 58].
The obtained sequences were examined using the Nucleotide BLAST program [ 25].
Chromosome 11 ESTs and DISC1 sequences were examined using the UCSC genome browser (http://genome.ucsc.edu).ucsc.edu
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The occurrence of chimeric sequences was examined using UCHIME, a part of the UCLUST package.
The set of 158 unique tRNA gene sequences was examined using cluster analysis.
To determine the evolutionary rates of the representative F/K sequences, substitution rate heterogeneity among coding sequences was examined using Tajima's relative rate test [ 60] by using MEGA4 with Bromus tectorum sequence (AY362757) as the outgroup.
The DNA binding affinity of SAHA conjugate 2 to its target sequence was examined using surface plasmon resonance.
The null hypothesis of symmetry in the distribution of gaps around the average of each sequence was examined using a sign test in SPSS 14 (SPSS Inc ., performed across all sequences.
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