Sentence examples for sequences were eluted from inspiring English sources

After washing, the binding sequences were eluted by heating, and the recovered ssDNA was used to perform negative selection using HCT 116.

The bound sequences were eluted in 500 µL BB by heating at 95°C for 15 min, cooled on ice for 5 min and centrifuged at 14,000 rpm for 2 min. The supernatant containing the DNA sequences was then incubated with a negative cell line to perform a subtraction of general sequences, as described above.

DNA not specific to these regions were washed off and targeted DNA sequences were eluted and amplified by emulsion PCR.

Bound sequences were eluted by heat into nuclease free water, reverse transcribed, amplified and cloned using the materials outlined for SELEX and cloning.

AhdI-fragments containing expression cassettes and selective marker flanked by δ-sequences, were eluted from an agarose gel and used for transformation of S. cerevisiae strains BY4742 and AS400.

DNA fragments containing simple sequence repeats were eluted from streptavidin-coated magnetic beads in a 1.5-mL tube and put in a freeze-drying machine at −40°C for 3 h.

All the distinctly upregulated, downregulated and uniquely expressed transcripts [ranging 50 400 bp] were eluted, sequenced and submitted to the EST database of Genbank.

Briefly, 454 sequencing libraries were synthesized, hybridized on microarray and subsequently, specifically hybridized library fragments were eluted and sequenced on a 454 GS-FLX sequencer.

PCR products were resolved through 1.2% agarose, and bands corresponding to SYT/S and SYT/L were eluted and sequenced.

The resulting cDNA product was loaded to a preparative agarose gel (1.5%) and fragments in a size range of 450 – 650 bp were eluted for sequencing.

Differentially expressed bands were cut out, PCR products were eluted, cloned and sequenced.