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The sequences were downloaded from Gramene53,54, Phytozome (http://www.phytozome.net) and Ensembl (http://www.ensembl.org).org
The DP protein sequences were downloaded from Dictybase (http://dictybase.org/) and the Physarum library was derived from the translated Physarum reference transcriptome11.
The rice protein sequences were downloaded from Gramene's Rice Ensembl genome.
Only a small part of identical sequences were downloaded in order to cover geographical range and substrate preferences.
As a first step, Blast searches were conducted in GenBank (http://ncbi.nlm.nih.gov), and closely related sequences were downloaded.
Similar sequences were downloaded and aligned with ClusterW and phylogenetic tree drawn with the MEGA 6 software.
Protein sequences were downloaded from IMG [40].
Plant MIRO sequences were downloaded from the NCBI database.
All the genome sequences were downloaded from NCBI database.
The AMP sequences were downloaded from CAMP [16].
Genome sequences were downloaded from the UCSC ftp site.
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