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Probes sequences were designed using Beacon Designer (Premier Biosoft, Palo Alto, CA, USA).
LRRK2 guide RNA sequences were designed using online target prediction (http://crispr.mit.edu/).edu/
These sequences were designed from exons 2 and 3 of the PTH transcript sequence deposited as NM_000315 in GenBank.
Primer sequences were designed for DNA regions where nucleotide variations were observed between the two morphotypes (Fig. 1, Table 1).
The specific primers to each one of the sequences were designed using GeneRunner 5.0 (Additional file 1: Table S1).
Several shots and sequences were designed to utilise the effect, such as the shark's destruction.
Primer sequences were designed using Primer3 (v.0.4.0) software.
Primer sequences were designed for each gene (Table S2).
Primer sequences were designed with Primer express software.
Primer sequences were designed to not contain CG dinucleotides.
The siRNA sequences were designed as described previously [70].
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