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Contiguous sequences were created from single sequence reads by using the CAP3 sequence assembly program (16 ).
Phylogenetic trees showing the nearest neighbor relationships of our sequences were created from these alignments.
All possible CATG + 17nt tag sequences were created from 24,955 full-length cDNAs and used as the reference tag database.
All possible CATG + 17-nt tag sequences were created from the Sus scrofa genome sequence (Sscrofa9.2) and UniGene (NCBI36.1, 20090827) databases and used as reference sequences to align and identify the sequencing tags.
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The HM database of expressed peptide sequences was created from the most extensive healthy human-microbial distal gut metaproteome available to date.
Ordination of the whole community detected by 16S amplicon sequencing was created from UniFrac matrix calculated by QIIME61 software and presented in a principal coordinates analysis plot.
"I think we're going to develop the next-generation platform together," says Ordoñez. *The Celera sequence was created from five individuals.
A consensus sequence was created from the most abundant amino acid at each position in the cohort.
A consensus sequence was created from forward and reverse sequences, and the genes were concatenated and queried against all 202 MLST sequences in the database (15 ).
Sequences for which pairwise similarity in the flanking regions exceeded 95% were grouped into contigs and a 2/3 majority rule consensus sequence was created from each contig.
The target DNA sequence is created from a given peptide sequence by the chosen codons; the codon encoding a given amino acid is chosen randomly with equal probability.
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