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Cytochrome oxidase I (COI) was sequenced for woodpecker species for which cytochrome b (cyt b) sequences were available.
The former three sequences were available at 30 fps.
All primer sequences were available in Table S1.
For most of the blocks, 29 sequences were available.
In addition, NUCB1 sequences were available for many species within Genbank and Ensembl databases.
DNA sequences were available for only a fraction of taxa potentially related to our target species.
However, 5'NCR sequences were available for only a fraction of 101 serotypes.
The concatenation of the 68 genes for which Convoluta sequences were available was done by the program ScaFos [32].
Of these genes, 53 5'-upstream sequences were available, only seven of which belonged to B-type RIFINs.
Only five potyvirus sequences were available to Nicholas and Laliberte and they based their designs on conserved amino acids.
Using organisms for which completed genome sequences were available, we created mixtures of cells with equal quantities of each organism.
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