Sequence data were generated using an ABI PRISM 3100 DNA Analyser (Applied Biosystems), and sequences were analysed using Sequencer 3.1.1 software (Applied Biosystems) to compare variations.
The bisulphite conversion rate was ≥98% and sequences were analysed using BiQ Analyser software [ 38].
In addition, the similarities between all analysed scFv sequences were analysed using a new on-line tool, which builds phylogenetic trees.
Sequences were analysed using an ABI Prism 3100 Genetic Analyser (Applied Biosystems).
The sequences were analysed using an automated DNA sequencer (ABI 3700; Applied Biosystems).
Sequences were analysed using an ABI Prism 310 automatic sequencer (Applied Biosystems, Foster City, CA, USA).
The sequences were analysed using a 3500 or 3130 Dx Genetic Analyser (Applied Biosystems, St. Aubin, France).
Sequences were analysed using the Geneious v. 6.1.6 software6.1.6
Sequences were analysed using mothur version 1.31.1 (ref. 33).
Sequences were analysed using the Sequencher® software (version 5.2.3; Gene Codes, Ann Arbor, MI, USA).
The DNA sequences were analysed using the Geneious software (version 7.1.3) described above.
