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The bisulphite conversion rate was ≥98% and sequences were analysed using BiQ Analyser software [ 38].
Sequences were analysed on an ABI Prism® 3900 DNA Analyser automated sequencer (Applied Biosystems).
Sequences were analysed on an ABI 3730 sequencer (Applied Biosystems).
Most sequences were analysed to genus level.
Sequences were analysed using the Sequencher® software (version 5.2.3; Gene Codes, Ann Arbor, MI, USA).
The DNA sequences were analysed using the Geneious software (version 7.1.3) described above.
The resulting sequences were analysed to verify the absence of mutations and reading frame conservation.
Primary sequences were analysed under neighbour-joining, maximum parsimony, maximum likelihood, and Bayesian approaches.
Viral loads were measured in samples with detectable HBV DNA and the DNA sequences were analysed.
Sequences were analysed by identifying overlapping regions between the partial fragments and the 5′ and 3′ RACE products.
Sequences were analysed using Seqscape v2.5 software.
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