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Kpn I- and Xba I-recognizing sequences were added to the ends.
"In the last 10 weeks, more sequences were added to the GenBank database than in the first 10 years of genomics," reveals Robbins.
These protein sequences were added to each pathogen database.
Shorter sequences were added to maximum-likelihood trees using the ARB parsimony insertion tool [69].
Using this protocol, 158 previously published sequences were added to the alignment (File S1).
Six nucleotide non-target sequences were added at the 5 prime ends of these primers (figure S2).
For each phylogenetic tree reconstruction, all selected protein sequences were added to a single multiple sequence alignment.
Primers were designed against the Gal4-luciferase operon in which the SalI-BglII restriction site sequences were added to the 5' end of the 5' primer.
Forty-seven prototype strain sequences were added.
These sequences were added to the dataset.
Further newly determined sequences were added in the same way.
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