Among our captured sequences, we identified some situations with up to 18 targets.
After further testing of shorter peptide sequences, we identified a CD8+ T cell epitope using cell lines expressing a relatively small number of algorithm-predicted candidate epitopes.
By analysing the entire promoter sequences, we identified 124 mouse L1 elements that contain CpG islands in their promoter regions.
In each of the 11 sequences we identified human-Fugu conserved TFBSs for a number of developmental regulators (Table. 1).
After removing overlapping sequences, we identified 55 USPs, 4 UCHs, 5 MJDs, 13 OTUs, and 12 JAMMs, which represented the 89 genes screened on TMAs (Table S2).
Among other available choanozoan cDNA library sequences we identified similar components from choanoflagellates and Capsaspora see also refs. [17] and [19].
Through the comparison of the human and chimpanzee draft genome sequences, we identified 252 inversions whose respective inversion junctions can clearly be characterized.
Visual and automated analysis ('network-clustering') of the Hox and Hox-like sequences we identified in Figure 3B revealed 7 major Hox-protein sequence similarity clusters (Figure 3C).
From alignments of whole FoxP2 gene sequences, we identified two regions of especially high variability that correspond to exon 7 (188 bp) and exon 17 (145 bp).
The sequence space we were interrogating comprised an entire genome, and the shRNA sequences we identified were selected from a random library, are unlikely to exist in nature, and may act by non-canonical mechanisms.
Screening for mutations in exons, exon-intron boundaries and regulatory sequences, we identified 14 single nucleotide polymorphisms (SNPs) in the PIK3R1 gene region from exon 1 to the beginning of the 3' untranslated region (UTR).
