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Since BAli-Phy is computationally intensive and generally considered to be too slow to be efficiently used with more than a dozen sequences, we conducted these analyses for both the ant and bee datasets independently.
To determine the number of viral particles necessary to generate full genome sequences, we conducted dilution series with viruses whose titer was determined by plaque assays.
To obtain quantitative estimates of the magnitude of selection acting on intron splice sequences, we conducted simulations using the ancestral selection graph (Neuhauser and Krone 1997).
After creating and analyzing a database of human antibody sequences, we conducted an in-depth analysis of the humanness of therapeutic antibodies, and found that increased humanness score is correlated with decreased immunogenicity of antibodies.
To validate our hypotheses that plant-infecting viruses are more likely to be targeted by plant miRNAs than by other sequences and that plant miRNAs preferentially target plant-infecting viruses over other sequences, we conducted the following analyses.
To determine membrane, nucleocapsid, nonstructural (Ns 7a, Ns7b, and Ns7c protein nucleotide sequences, we conducted PCR and DNA sequencing in the same manner as for determination of partial RdRp nucleotide sequence described above by using the HKU9-Leader42 64 primer (5′-CCGTTTCGTCTTGTACGAATCAC-3′) and the 3siteAd20T primer (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTTTTTTT-3′).
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To provide an overview of the current health economic evidence for genome sequencing, we conducted a thorough systematic review of the literature from 17 databases.
Before we amplified and subjected patient samples to deep sequencing, we conducted duplicate control experiments to assess the effects of PCR amplification and deep sequencing with 454 technology on amplicon quantification and error rates using 3 clones from subject 07 at an input ratio of 89∶10∶1.
For each spruce sequence, we conducted three blastp searches against the protein datasets from Arabidopsis, rice and pine.
To assess the amount of nucleotide differentiation among the six Penstemon species sequenced, we conducted pairwise BLAST searches of all the 454 contigs assembled for each species.
By using ultra-deep sequencing, we conducted a thorough assessment of HCV-NS3 protease variants in chronic PI-naïve patients infected with HCV-1a and HCV-1b under telaprevir-based triple therapy at baseline and after 4 weeks of treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com