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Gametic phase of POMC sequences was determined computationally using PHASE [ 56], and haplotype determinations from the best pairs output were used.
The location of the open reading frames (ORFs) in the sequences was determined using FramePlot version 2.3.2 (http://www0.nih.go.jp/~jun/cgi-bin/frameplot.pl) (Ishikawa and Hotta 1999).
The concentration of M- and H-centred sequences was determined experimentally from the analysis of the α-CH3 resonance signals of M and H units, which gave a relatively complex pattern assigned to M and H centred triads with specific composition and stereochemical configuration.
The clonal nature of the Comenzo sequences was determined in a similar manner.
The relative activity of 3CLpro against these substrate sequences was determined (Figure 5).
Additionally, about 2,400-bp 2,400-bpsequencesermined in twasother strains (6V, 7V).
The presence of Wolbachia wsp gene sequences and mitochondrial 12S rRNA gene sequences was determined in each extract by PCR.
quinquefasciatus EDS39158 sequences was determined by subsequent expression (Table S1) and biochemical analysis of their recombinant proteins (not shown).
The GC content of the upstream and downstream sequences was determined in the different Pseudomonas whose genomic environment was studied.
To calculate the relative abundance of transcripts representing each of the rups groups, the percentage of cloned rups related sequences was determined for all stages.
After 24 hour of incubation, the efficiency of the different GRP78 siRNA sequences was determined by Western blot analysis using anti-GRP78 specific antibody (Fig. S2).
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