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Initial filtering of obtained sequences was performed using quality information.
GSAT MM sequences was also enriched in H3K9me3 ChIP-seq.
Mean length of retained sequences was 269 bp.
Pre-assembly trimming of sequences was performed with Phred53.
A decoy database of reverse sequences was used to report 1% peptide false discovery rate (FDR).
A neighbor-joining tree of these sequences was calculated with SEMPHY74.
A set of 48,298 target amino acid sequences was announced in September 2010.
The quality of the 1 × 75 bp sequences was checked using FASTQC (version 0.10.1 61.
Local alignment of protein sequences was done using the FASTA suite (http://fasta.bioch.virginia.edu/fasta_www2/fasta_down.shtml).shtml
Alignment of selected DNMT3, DRM and DNMT1 MTD protein sequences was performed using MUSCLE v3.8.3164.
Their ability to repeat those sequences was then tested at various intervals, including after one and two nights of sleep.
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