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In most cases, the bFLICs span the entire CDS of the genes, providing the basis for creating predicted bovine protein sequences to support proteomics and comparative evolutionary research as well as functional genomics and genome annotation.
All of these libraries have been end-sequenced to support BAC-by-BAC sequencing and extension, and to provide a base of genome-wide survey sequences to support studies such as the one presented here.
We have used 3945 compositional properties including frequencies of mono, doublet, triplet, and multiplets of amino acids and hydrophobic properties as input features of protein sequences to Support Vector Machine.
In order to circumvent this problem and generate a large set of full-length coding sequences to support the genome sequencing and annotation in catfish, doubled haploid catfish was produced that has been demonstrated to harbor two sets of homozygous chromosomes [ 16].
Thus, the co-coexpression analyses are very interesting indicators to identify hypothetical Plasmodium genes that have sequence and expression similarities with a yeast and/or Drosophila functional gene, but they cannot entirely replace an expert examination of the sequences to support or reject the possible annotation transfers they propose.
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An additional long-range barcoded DNA library (Chromium system by 10x Genomics) was sequenced to support haplotyping of heterozygous genomes, scaffold validation and further longer-range extension of the scaffolds.
However, the process illustrates one mechanism by which retrogenes could be brought into close proximity to prefabricated promoter regions, gaining the sequences necessary to support transcription.
The results of molecular phylogenetic analyses of cytochrome b mtDNA sequences fail to support our initial hypothesis that mouse lemurs collected at Beza Mahafaly with murinus-like or unique pelage characteristics are either M. murinus or a novel species.
The fact that some datasets, especially the atp8, nad1 and nad4L sequences appeared to support multiple topologies probably reflect an overall lack of phylogenetically informative sites in these genes (Additional file 1: Figure S4).
We first performed 454 GS-FLX sequencing because there was no genomics information available for the species, and then used Illumina sequencing to validate those results, and to provide greater sequencing depth to support a differential gene expression analysis.
Unlike amplicon sequencing, the various current methods for GBS are not restricted by a need for prior sequence knowledge to support primer design, and can potentially generate hundreds of thousands of marker loci [ 58, 59].
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