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Most importantly, it may be the organism of choice for reliable, large-scale production of heterologous membrane proteins, using inducible intracellular membranes and targeting sequences to specifically insert these proteins stably into these membranes.
Naldini and colleagues were the first to use miRNA target (miRT) sequences to specifically suppress transgene expression from lentiviral vectors in hematopoetic cells or hepatocytes [6], [7].
Pairs of PCR primers (Table S3) were designed from an alignment of the I. ricinus IxAC coding sequences to specifically amplify each of the family members one at a time, each primer pair generating a product of different size.
Cationic liposomes carrying plasmid or CpG DNA have been used as adjuvants to specifically enhance immunity against infection and tumors [60], [61].e carefully screened siRNA sequences to specifically avoid the Interferon response [64], [65] and used liposome concentrations well below those used as adjuvants to prevent immunogenicity.
The heptapeptide motif was selected from a library of random sequences to specifically bind HIV-1 regulatory proteins Tat or Rev.
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The entire genome sequence was determined in the H5N1 isolate from the cat, while the H5N1 isolate from the pigeon was sequenced to specifically obtain the HA, neuraminidase, and PB2 genes.
Aligning cDTs that are specific to the CNS or PNS may indicate sequences required to specifically drive expression in either the CNS or PNS.
Two different miRNA sequences were used to specifically target the coding sequence of human SOD1: miR SOD1 (target sequence: nt. 209-229, NM_000454.4) and miR-b SOD1 (nt. 265-285) were selected for in vitro and in vivo assessments of silencing efficiency.
NF-κB then targets specific DNA sequences to facilitate gene expression, specifically of genes critical in cellular death.
Molecular barcodes with multiplex identifiers (MIDs, unique 10 bp sequences) were applied to specifically tag each cDNA library in our pooled sequencing run.
In conclusion, the ten isolated promoter sequences were confirmed to specifically drive gene expression in meiocytes.
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