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The foreseen application is a good training ground to evaluate the proposed technique and the effectiveness of this work has been demonstrated through experimental results with endoscopic image sequences to efficiently locate boundaries of a landmark-free needle-holder at half the video-rate.
Resources such as the RNAi Consortium (http://www.broadinstitute.org/rnai/trc) and RNAi Codex (http://cancan.cshl.edu/cgi-bin/Codex/Codex.cgi) increasingly facilitate the selection of gene-specific siRNA sequences to efficiently knock down gene function.
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Future efforts to characterize the Giardia transcriptome would benefit from using high throughput sequencing to efficiently enhance the resolution and confirm the expression of proposed gene models.
Mobile Element Scanning (ME-Scanning) relies on targeted high-throughput sequencing to efficiently read nearly all the junctions between insertions of a class of mobile elements and the genomic flanks of those insertions.
The W-curve does not require gap-stripped, reduced sequences to run efficiently.
TRIMs of plants and ants are presumed to be the result of convergent reductive evolution from autonomous elements, retaining only the minimal sequences required to efficiently complete the retrotransposition cycle (Schulman 2012).
We presented a strategy for using short sequences derived from second generation sequencing technology to efficiently identify large numbers of SNPs with MAF estimates at a low false discovery rate.
In conclusion, we have demonstrated the use of short-reads sequence data to efficiently and comprehensively characterize a draft transcriptome of an organism without sequenced genome.
Our group continues to lead the field, and we're now applying NextGen sequencing methods to efficiently identify ENU-induced mutations.
We have demonstrated the use of a high-throughput sequencing methodology to efficiently produce a snap-shot of transcriptional activities occurring in the first larval stage of C. elegans development.
Following the detection of a siRNA sequence able to efficiently interfere with COX-2 expression in HUVE cells, we focused our attention on a new RNAi strategy based on the use of a shRNA-expressing vector.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com