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To identify peptide sequences that recognize and bind to ZP proteins, we developed a novel selection procedure from phage display libraries that utilizes intact oocytes surrounded by ZP proteins.
As the selected pools were enriched with sequences that recognize and bind to the target cell line, an increase in fluorescence signal was observed (Figure 1a).
To this end we used THREADER 3 to analyze the percentage of sequences that recognize the correct structure using different models.
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This result is in agreement with an earlier analysis based on a combined dataset of LSU and SSU sequences that recognized the Platyzoa as a derived monophyletic assemblage within the Lophotrochozoa [ 23].
These sequences cooperate with factors that recognize natural splice sites, whose sequences and relative strengths can vary considerably.
Breakpoints were cloned by long range PCR using primers that recognize sequences flanking the distal and proximal breakpoints, respectively, based on the customized array-CGH data.
In addition, all deletions were confirmed using TaqMan assays [ BMP4 probes Hs02617539_cn (5′) and Hs00282899_cn (3′) that recognize sequences located within the BMP4 first and last exons, respectively, and OTX2 probe Hs01323383_cn designed against OTX2 exon 3 (first coding exon) sequence].
The current prediction method is based on our classification of the RNA editing site neighbor sequences that may reflect similarities in the trans-factors that recognize those sequences.
First, consider a pure sequence interactor that recognizes only the sequence of its target DNA.
This model predicts that functionally related genes are regulated posttranscriptionally as groups by specific mRNA binding proteins that recognize sequence elements in common among the mRNAs.
We initially hypothesised that two individuals infected by parasites sharing at least one identical VB sequence should present IgG that recognize the DBL6ε domain.
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