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We have developed a neural network method to predict the initiation site of mRNA sequences that lack the preferred nucleotides at the positions −3 and +4 surrounding the translation initiation site.
We know that coding sequences that lack transcription signals can be activated by the upstream insertion of mobile elements in bacteria and yeast.
Sequences that lack crucial functional residues in the catalytic domain are discarded.
This is consistent with our findings in this study, since a phylogenetic analysis using only sequences that lack Latin names would be difficult to root in any general standard of measure.
Interestingly, sequences that lack C2 and C5 cysteines were observed in the alignments.
Disordered protein regions are protein sequences that lack a fixed three-dimensional structure under putatively native conditions.
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Similarly, all short sequences and sequences that lacked the ITS2 subregion were excluded.
Among the incorrect constructs, 22% contained sequences that lacked inverted repeats, 6% contained an incorrect loop sequence, and 2% lacked the poly-T transcription termination sequence.
The results revealed that the integration product copy number into the target sequence that contained both CA and GT was significantly greater than the copy numbers into sequences that lacked CA, GT, or both (Fig. 3A, B).
There were 4759 sequences that lacked metadata relating to isolation source.
We also removed sequences that lacked two PROSITE (http://prosite.expasy.org/scanprosite/, PS50090) R repeats [ 14, 72].
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