First, the team created DNA primers to amplify short mitochondrial sequences specific to bullfrogs.
We designed a series of DNA aptamer sequences specific to doxorubicin, displaying multiple binding sites and various binding affinities.
The hydrolysis of peptide bonds was studied in incubated solutions of proteasome with oligopeptide sequences specific to each chymotrypsin, trypsin and caspase activity of proteasome, before and after inhibition with epoxomicin.
The repeats in O. coarctata are quite diverged from O. sativa, so much so that O. coarctata has a higher amount of unique repetitive sequences specific to its genome.
Sequences specific to SG 9R, which are absent or highly divergent in the fully sequenced biovar Gallinarum strain 287/91, were identified by constructing the suppression subtractive hybridization (SSH) library.
The mitochondrial cytochrome oxidase subunit 1 (COI) nucleotide sequences of 29 B. pulchra, 157 skates and rays reported in GenBank DNA database were comparatively analyzed and the COI sequences specific to B. pulchra was identified.
The assays utilize an upstream primer that is derived from 3′ end of the genetic element IS 711, whereas the downstream primers and probes are designed from signature sequences specific to a species or a biovar.
To facilitate diagnosis of the pathogen, we designed SCAR primers capable of amplifying DNA of E. lata and constructed a genomic DNA library from which DNA sequences specific to E. lata were identified and sequenced.
Primer sequences specific to 12 of target genes were generated with Beacon Designer software (Bio-Rad).
Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative oligonucleotide probes were selected.
From the analysis of sequence polymorphisms, it is difficult to determine in which form the mutation first occurred because there were no sequences specific to one form.
