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The bioinformatics program QDD (Meglécz et al., 2010) was used to analyze the raw sequences, select the sequences with target microsatellites, and perform primer design.
From the web interface, researchers can upload a file of sequences, select the search algorithm (e.g. BLAST, FASTA), the database (SSR sequences, SSR primers) and submit their job for processing.
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Fig. 8 Modeled sequences selected from group 1 and group 2 for forecasting event B7.
Representative sequences selected from each OTU were then used to build phylogenetic trees.
Three species and the sequences selected were barramundi Lates calcarifer (accession no.
The new experimental design is a set of stacking sequences selected from candidate stacks using D-optimality.
As a result, promising management sequences selected with the model underestimated the importance of selling small fruits at local market.
The sequence space is initially searched by Monte Carlo sampling guided by a public atomic potential, with candidate sequences selected by the clustering of sequence decoys.
To confirm that this second set of primers was amplifying nematode sequences, selected bands on the DGGE gels were extracted, PCR amplified and sequenced.
In this study, we have evaluated a spoligotyping membrane prepared with 25 novel spacer sequences selected from a previous study [van der Zanden AG, Kremer K, Schouls LM.
With the four highly specific sequences selected, we designed primers to be used in the MLS-PCR to directly genotype T. cruzi from biological samples.
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