Sentence examples for sequences per cell from inspiring English sources

We evaluated the copy number of HER-2/neu sequences per cell using a dual-colour FISH assay which included the gene probe and the centromere probe as a control.

Relative copy numbers of oriC and terC sequences were determined by the ΔCt method: the difference in amplification rates of oriC and terC (ΔCt) for each sample (average of four replicate reactions) were normalized to the ΔCt for a wild-type strain grown to saturation (stationary phase) shaking in LBH at 37°C for 28 hr, to contain equal numbers of oriC and terC sequences per cell.

Monoclonality of memory B cell lines that were cultured at clonal density (Table 1) could be confirmed by VDJ sequencing per cell line, as only one VDJ sequence per cell line was detected (Figure 4).

The detection threshold is 2 copies of target sequence per cell.

Positive signals were observed in the nucleus despite of low copy number cccDNA (2 copies of target sequence per cell).

RCA combined with IS-PCR yielded strong positive signals in HCC liver tissue in spite of low copy number cccDNA (2 copies of target sequence per cell), meanwhile, no positive signal was detected via negative control.

After transformation by B77 virus or by Prague C strain of RSV, duck cells contain four to six copies of RSV DNA sequences per diploid cell [ 14].

Determination of the number of complements of labeled virus-specific sequences and of diploid-cell genomes in each reaction mixture permits calculation of the copy number for those viral sequences per diploid cell.

These cells, like most of the other RSV-transformed mouse, rat, or hamster cells we have studied [ 5, 8], contain one to two copies of RSV-specific DNA sequences per diploid cell.

Figure  5 displays the equal effects of unfractionated, network, and supernatant DNA from RSV transformed duck cells upon the reassociation of slowly reassociating DNA, consistent with the presence of four copies of RSV sequences per diploid cell.

It further circumvents the limitations of previously used transient transfections (Melnikov et al., 2012; Patwardhan et al., 2012; Sharon et al., 2012; Arnold et al., 2013), which lack a proper chromosomal context and are not controlled for amount of sequence variants per cell and their copy number.