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These methods will facilitate investigations of whether miRNAs interact with sequences outside of 3′-untranslated regions and suggest new targets for the design of synthetic modulators of gene expression.
This suggests not only that sequences outside of the C-terminal region may also contribute to the interaction with co-receptors but also that FGF23-21c may be able to regulate both glucose and phosphate metabolisms.
Briefly, to introduce random mutations into the phaC gene, forward and reverse primers (ReCMutF; 5′-GATCCCCCGGGCAAGTACC-3, ReCMutR; 5′-GGGAACCTGCAGGCCTGC-3′) were designed based on the nucleotide sequences outside of the structural gene.
By contrast, sequences outside of transcriptionally important regions showed high variability [9].
The specificity (or the false discovery rate) of the two systems was evaluated by looking for perfect matches with sequences outside of the Firmicutes and Bacteroidetes phyla.
For the HML-HIS4 interval, we used the S. cerevisiae sequences outside of HML to the nucleotide before the stop codon of HIS4 (chromosomal co-ordinates 14850 65933).
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Candidate sequences which did not permit the placement of at least one PCR primer within unique sequence (ie outside of a repeat identified by Repeat Masker) were not tested further.
The relative amount of RNA produced in these experiments varied with the DNA sequence outside of the miRNA stem sequence.
Drug-resistant clones were screened by Southern blot analysis using PstI- and SpeI-digested genomic DNA hybridized, respectively, with a 0.7-kb 5' genomic fragment corresponding to a genomic sequence outside of the sequence used in the targeting vector and a 0.6-kb PstI PGK-neo probe (Fig. 3A).
LSGs are defined on the basis of displaying no significant sequence similarity to any other peptide sequence outside of the lineage.
We used the eukaryote setting with each sequence truncated after 70 residues to avoid false positive detection of signal sequence outside of the N-terminus.
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