After two successive rounds of capture, >90% of the resulting cDNAs were GH11 sequences, of which 70% (38 among 53 sequenced genes) were full length.
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This gave 4 output sequences, of which two lie in non-coding regions.
(d) Sequences without poly(A) tails: all sequences of which no polyadenylation signal or tail was found.
In the Vβ6.1 pool, 60 clones were sequenced, revealing 12 unique sequences of which 4 were Jβ2.7+.
At the bottom of the phylogeny, clade V contains 24 sequences of which none have been biochemically characterised to date.
A set of 11 PCR primer pairs (Table S2) was based on the pol and nap mitotype sequences, of which eight (P3-9, P11) are pol-specific.
A total of 52,571 distinct sequences were identified from the raw sequences, of which only 13,782 mapped to the human genome reference sequences.
The 28S data matrix includes 108 sequences of which 80 are new and 9 are lengthened since Cárdenas et al. [35].
The reads assembled into 45,538 contigs (here, a "contig" is a set of contiguous sequences), of which, 11,918 shared homology to existing protein sequences.
The second most represented taxon was the stramenopiles, accounting for 20% of the eukaryotic sequences (of which 10.9% were MAST, marine stramenopiles).
