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The typical consensus sequences of Type I BVMOs are well conserved in BVMOLepto (Fig. 1b).
Sequences were compared against sequences of type strains using RDP release 11 (https://rdp.cme.msu.edu/).msu.edu/
The nucleotide sequences of type strains were retrieved by the National Centre for Biotechnology Information (NCBI www.ncbi.nlm.nih.gov).nih.gov
Also, a motif NK /I PGR /K) is present in the sequences of type II DAHPS including At-DAHPS.
The two Rossmann-fold motifs (GxGxxG/A) and the two consensus sequences of Type I BVMOs (G/AGxWxxxxF/YPG/MxxxD and FxGxxxHxxxWP/D) are written in bold.
This cleaning allows recommending sequences of type A→B→C→(X), where (X) is, in turn, a path containing one or more tasks, to be recommended to the user.
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To amplify regions of HAdV-D53 flanking the sequences described by Engelmann et al. [19], we designed primers based on conserved adenovirus sequences of types in HAdV-D.
The 145, 44, and 169 sequences of types ABC, ABD, and ABCC, respectively, from strains with disease information were used for phylogenic analysis with ClustalW (http://align.genome. jp/).
Both flanking regions contain the recognition sequences of type-IIs restriction enzymes BbsI and BbvI, respectively.
Unfortunately, at present, there is no general method for determining recognition sequence of Type I or Type IIG RM systems.
For example, a sequence of type A has only one EPIYA segment A and a sequence of type ABCCCCC has seven EPIYA segment, including five repeats of segment C. The majority (74% = 416/560) of sequences had three EPIYA segments.
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