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In addition, sequences of transcripts produced by the reference isolate (BFP-ToxAC), the sequenced nonpathogenic isolate (SD20-NP), and an additional pathogenic isolate (SO3-P) were analyzed.
The appropriate Cy3 and Cy5 labeled probes were combined and used to probe the processed mouse oligo microarray chips (approximately 36,000 sequences of transcripts).
Sequences of transcripts and chromosomes were obtained for S. cerevisiae and H. sapiens from the Ensembl database (www.ensembl.org), A. thaliana from the Arabidopsis Information Resource (http://www.arabidopsis.org/) and D. melanogaster from FlyBase release 5.4 (http://flybase.bio.indiana.edu/).edu/
Sequences of transcript-specific primers used for transcript validation and tissue-specific expression analysis are given in Additional file 1.
The amino acid sequences of transcript-deduced amino acid sequences of PLA2 molecules, serine proteinases, and SVMPs are shown in Additional file 3: Figures S1-S3).
Additional file 1: Sequences of transcripts (fasta seqs) comprising MSGI 1.2 assembly.
Sequences of transcripts listed in table 4 were identified from each assembly.
Sequences of transcripts were translated into amino acid sequences by BioEdit and further aligned using MUSCLE ver. 3.6 [ 62].
All of our abundances were based on alignments of reads against only the coding sequences of transcripts.
EST projects often aim to obtain the five prime end sequences of transcripts in order to characterize protein-coding regions.
3USS also permits to easily retrieve the genomic coordinates and sequences of transcripts already annotated in public databases.
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