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The genealogy with its defined mutation positions defines the SNP haplotype sequences of the sample.
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Different from hybridization-based microarray technologies [ 3, 4], sequencing-based technologies do not require prior information about the genome or transcriptome sequences of the samples of interest [ 5].
The percentage nucleotide identities between the cox1 gene sequences of the samples and those of the corresponding best match sequences in GenBank were 98 100%.
According to the SILVA taxonomic database, all sequences of the samples were classified from phylum to species by using the QIIME program.
Secondary structure protocol sequencing was required in order to obtain complete sequences of the samples.
Homologous DNA sequences of the samples from the USA and India were downloaded from Gen Bank.
Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains.
The M gene sequences of the samples 281 01 and H03 2080, however, were only 71%to82%2% identical to any other known bornavirus sequence.
More importantly, these methods tend to emphasize mutations as the major events that cause the differences in the DNA sequences of the samples.
†Nucleotide/amino acid sequences of the samples recovered from a dump at Istituto Sieroterapico Milanese (ISM), Milan, Italy, and analyzed in this study.
The adaptors provided priming sequences for amplification and sequencing of the sample library fragments as well as 'the sequencing keys', a short sequence of 4 nucleotides.
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