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Using overlapping PCR products, we confirmed the flanking intronic sequences of the coding exons and that the entire 466-residue mature peptide is encoded by the last exon of the gene.
The conservation of sequences of the coding region of structural proteins of different WSSV isolates provides a strong basis for generating highly specific and sensitive nucleic acid and immuno-based molecular detection methodologies for WSSV (You et al. 2004).
The beginning and end sequences of the coding regions of the sequences listed above are conserved (Figure S5).
We compiled the sequences of the coding regions of PCSK9 from 14 primate species in the clade of Hominoids, Old World monkeys and New World monkeys.
Protein sequences and DNA sequences of the coding regions ranging from daf-2, age-1, pdk-1, akt-1, and daf-16 were obtained from WormBase WS197 (www.wormbase.org).org
The DNA sequences of the coding strand of the double-stranded oligonucleotides used for this study are listed in Table 2. Electrophoretic mobility shift assays (EMSAs) were performed as described previously [24].
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Sequencing of the coding and anti-coding strand of the oprD PCR products necessitated two additional internal primers (Table 6).
This study involved cloning and sequencing of the coding regions of all 10 Toll-like receptor (TLR) genes of goat.
Comprehensive sequencing of the coding regions of all genes (Whole Exome Sequencing or WES) is more affordable, but still has high technical requirements that are an obstacle to its use as a diagnostic method in routine clinical practice.
The amino acid sequence of the coding region corresponds to a protein of 784 amino acids long with predicted molecular weight 104 kDa and 6.97 pI value.
Full-germline DNA sequencing of the coding regions was performed on three HNPCC genes, MLH1, MSH2 and MSH6.
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