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Genomic DNA sequences of target genes were analyzed with the software ZiFiT Targeter (version 3.3) to identify ZFN sites for which ZFNs could be engineered using the Context-Dependent AssemethodCurtinmethod (Curtin et al. 2011; Sander et al. 2011).
Antisense oligonucleotides (AOs) have been designed against splicing regulatory sequences such as splicing enhancer sequences of target exons.
One of the PCR primers was 5′-phosphorylated to obtain the duplex products in which all antisense sequences of target DNA would contain phosphate groups at the 5′-ends after PCR amplification.
Reverse complement sequences of target (red) and PAM (shadowed) are shown on the reference sequence.
In most cases, these transcription factors exert their actions by directly binding to cognate consensus sequences of target genes (Li et al., 2015).
The evolution over time of the target's kinematic and aspect states is described then by a coupled stochastic dynamic model where the sequences of target positions, velocities, and aspects are mutually dependent.
MicroRNAs participate in post-transcriptional regulation of cellular differentiation by binding to the complementary sequences of target messenger RNAs (mRNAs) or non-coding RNAs and down-regulate their target gene expression.
Primer sets specific to approximately 100 bp sequences of target genes and controls (PUM1 and GAPDH) were ordered from Invitrogen.
PCR primers were designed using nucleotide sequences of target genes identified in the M. graminicola genome project (Table S2).
In E. coli, RyhB binds to complementary sequences of target mRNAs, causing their degradation in an RNaseE- and Hfq-dependent manner [23], [24], [25].
Within the nucleus, the ligand-PPAR-γ complex binds to PPAR response element (PPRE) DNA sequences of target genes, and positively or negatively regulates their transcription [11].
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