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Table 1 Sequences of study treatments 1. PCF NAP PLA 2. NAP PLA PCF 3. PLA PCF NAP 4. PCF PLA NAP 5. NAP PCF PLA 6. PLA NAP PCF PCF paracetamol 1,000 mg + caffeine 130 mg, NAP naproxen sodium 550 mg, PLA placebo.
Participants were randomly assigned to one of four sequences of study medication (ABAB, ABBA, BAAB, or BABA, where A=prednisolone and B=placebo).
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GenBank accession number of nuclear gene (rag1, rag2, tmo4c4, zic1, myh6, and btbd7) sequences of studied species.
Primer sequences of studied genes are presented in supplementary data files (see additional file 6 for trout; see additional file 7 for Xenopus).
We created a ribosomal RNA (rRNA) and transfer RNA (tRNA) database using BLASTn (e-value threshold of 10-4) for the 16S, 23S, 5S rRNA, and tRNA gene sequences from NCBI Genbank against the complete genome sequences of studied bacteria.
Sequence of study procedures are illustrated in Figure 1. 1 NA: Not Applicable.
Study investigators assigned the study drug sets to participants consecutively according to the sequence of study entry.
(iv) We utilized a quasi-randomization procedure based on time of practice attendance and fixed sequence of study groups.
For each outcome, a repeated-measures ANOVA taking into account the sequence of study interventions was fitted.
For each level of strata, a random sequence of study group assignments is generated using a computerized random-number generator [ 12].
We chose a fixed sequence of study conditions to avoid counselling activities of the practitioner in the first and second weeks.
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