In other situations, it seems that the similarity-controlled shuffling is able to create sequences of segments that are realistic.
As shown in Figure 2, the lengths of segments AC and AD are the same and the sequences of segments AC and AD are very similar.
The sequences after the stretch of amino acids, QVAKKV, in segments BC and BD were highly variable, while the sequences of segments C and D were completely different.
The coding sequences of segments 7R and 11R are identical to those of the wt segments 7 and 11 of virus M0, while segment 7RΔ has a modified ORF encoding an almost full-length duplicated NSP3 protein (mNSP3) [16].
The coding sequences of segments 7R and 11R are identical to those of the wt segments 7 and 11 of virus M0 .. Segment 7R contains a full repetition of the NSP3 ORF.
Although these point mutations occur in exon segments, they may not explain such a distinct change in the sequences of segments.
A total of 63 complete sequences of segments 2 of H5N1 isolated in 24 different Asian, European, American, and African nations from 1959 to 2012 were included.
The complete sequences of segments 1-11 of SMReV were obtained and have been deposited in GenBank under accession numbers HM989930- HM989940.
