Sentence examples for sequences of inserts from inspiring English sources

All generated recombinant plasmids were sequenced (GATC, Konstanz, Germany) to ensure correct orientations and sequences of inserts.

The nucleotide sequences of inserts were verified by automatic sequencing.

The sequences of inserts of pGL3-T and pGL3-G were confirmed by direct sequencing.

Therefore, we constructed a DNA library containing inserts averaging 3 kb in length and analyzed the end sequences of inserts using ABI3700 for ordering of contigs (Additional file 1).

The sequences of inserts and fusion sites with 35Smin and the GUS reporter gene in pCambia1281X and pBi101.1 vectors were verified by sequencing for 3x FORCA- pBT10, 3xM5-pBT10, 3x FORCA-pCAMBIA, 3xM5-pCAMBIA, 3x FORCA-pBi101 and 3x FORCA-mut-pBi101.

In the second step, a DNA library in which the average size of inserts was about 3 kb in length was constructed and the end sequences of inserts were analyzed using the ABI3700 sequencing system (Applied Biosystems, Foster City, USA) in NICEM (Seoul, Republic of Korea; Additional file 1).

Recombinant molecules were isolated from clones and sequences of inserted genomic DNA fragments were obtained by cycle sequencing followed by electrophoresis on an ABI 3730 sequencer (Applied Biosystems).

To determine the sequences of inserted DNA, the recombinant plasmids were isolated from the clones, and sequenced by dideoxy-chain termination method at Invitrogen Ltd. (Shanghai, China).

We verified that the sequences of inserted approximately 60 nucleotides (about 20 nucleotides of miRNA and about 40 nucleotides added at the reverse transcripts) were all correct, and could not find pre-miRNAs inserted into the vector.

Appropriate frame and sequence of inserts was confirmed using an ABI Prism 3730xl DNA Sequencer (Genewiz, South Plainfield, NJ, USA).

Colony PCR and DNA sequencing of inserts from recovered clones after ligation and transformation demonstrated an even distribution of construct sizes (Fig. 1B).