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It would therefore be prudent to provide more evidence for an environmental persistence of S. flexneri, for example from genome sequences of food or environmental isolates.
The results gained via the observations and the questionnaire show a certain amount of inappropriateness during food preparation processes between groups, especially in terms of poor hygiene, incorrect sequences of food preparation, incorrect temperature and distribution of food in the refrigerator.
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With regard to the initial sequences of feeding behavior, no between-food differences were observed.
We hypothesized that food availability and the sequence of food ingestion influence intake of PSCs and nutritious foods by sheep because PSCs increase need for nutrients.
During learning of the alternation task, the rat must integrate reward information with memory of the recently-visited sequence of food wells [65] [67].
These transition times were used to reconstruct the sequence of food-well visits and the durations of those visits.
Food intake requires a well-ordered sequence of searching, food getting, and ingestive activities.
We recovered PMMoV RNA sequences from 57% of food products containing pepper or spice and purchased from grocery stores in Marseille (southeastern France), indicating that these products represent a common source of viruses through ingestion.
The genome sequence of a food isolate (F6854) from the 1988 episode has previously been determined using automated Sanger sequencing [ 28].
The coding sequences for all genes annotated in the comK prophage in the 2000 isolates (J2818 and J0161) were used as queries in blastn searches against (i) the genomic sequence of F6854 19888 food isolate); (ii) the Listeria database at the Broad Institute website [ 42]; and (iii) GenBank [ 41].
If crows still extracted tools and probed into the food container when food was not present, it would be difficult to conclude that successful sequences of behaviour when food was present were the result of goal-directed planning.
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