This study demonstrated the significance of oriented ligand presentation for MSC chondrogenesis, and the importance of facilitating an orderly sequence of differentiation events, initiated by proximal interactions between MSCs and the extracellular matrix for robust neocartilage formation.
During their thymic development, T lineage committed precursors progress through an ordered sequence of differentiation events [2].
Despite this, the phylogenetic topology showing O. latifolia as the sister lineage to the clade composed of O. alta and O. grandiglumis [4], [71] is consistent with the temporal sequence of differentiation inferred from herbarium data.
Assuming the same sequence of differentiation in Hassall's corpuscle-like structures, the few K10+/eGFP− and K10−/eGFP+ cells in the thymic medulla would not represent an entirely distinct cell population but mTEC at corresponding intermediate stages of differentiation.
Thus, rather than the single high Yan progenitor state previously modeled, our results suggest a dynamic range of high Yan states from which different cell fates are specified according to the spatio-temporal sequence of differentiation.
A central player among the signals that coordinate this complex sequence of differentiation events is the secreted glycoprotein Reelin, which also modulates synaptic plasticity, learning and memory formation in the adult brain.
In addition, several unique signature sequences for differentiation of B. mallei and B. pseudomallei were identified.
Other investigators have used PCR-based methods targeting intronic sequences for differentiation of C. albicans from C. dubliniensis[ 25- 27].
This directed RSC differentiation follows the temporal sequence of photoreceptor differentiation in vivo, and the cells exhibit morphology, protein and gene expression consistent with primary cultures of rods in vitro.
