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A conjecture of Golay [19], that the highest aperiodic merit factor for sequences is achieved by Legendre sequences of length p at shift ( frac{p}{4} ), is now extended to two dimensions, where the maximum MFa is obtained for Legendre p × p arrays occurs at or near shift ( left(frac{p}{4}, frac{p}{4}right) ).
We were aware that the best accuracy in reconstruction of ancestral protein sequences is achieved at the identity level of not less than 70% [ 60].
The study of both methylated and non-methylated sequences is achieved by the design of primers that target either the methylated or non-methylated regions followed by methylation-specific PCR [ 76].
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After trimming, a total of 140,756 Expressed Sequence Tag sequences were achieved.
The eletropherograms were analyzed and consensus sequences were achieved with CodonCode Aligner v.3.0 (available at http://www.codoncode.com/).
Gap closure of the sequences was achieved by PCR amplification with the following primer combinations: trfA_F and ssb_R, trbB_F and trbC_R, korC_R and kleE_1F, 11390_F and 11395_R, trbN_F and res_R, res_F and zetaR.
Serotype identification of the sequences was achieved by comparison with Genbank data using the Basic Local Alignment Search Tool BLASTT) [ 42].
Alignment between snoRNAs and the above mentioned RNA sequences was achieved using a modified BLASTN program.
The alignment of these sequences was achieved using MUSCLE v.3.8 [ 47], as implemented in SeaView v.4 [ 55].
Out of 3,264 total sequencing attempts (large exons required sequencing several overlapping PCR products), 3,076 successful sequences were achieved.
Multiple-sequence alignment of amino acid sequences was achieved with ClustalW program http://www.expasy.ch/tools/ align[ 43].
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