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These components contain binding motifs which are specific peptide sequences interacting with integrin on cell membranes (Giancotti and Ruoslahti, 1999; Stupack and Cheresh, 2002).
As this method is based on the unbiased identification of sequences interacting with specific baits, there are unique opportunities for unravelling the secrets of how the genome functions in 3D.
Lastly, steroid receptor sequences interacting with signaling effectors or scaffolds represent potential therapeutic targets to specifically inhibit signaling effectors involved in hormone action.
We found long-lasting alterations in DNA methylation as a result of fetal alcohol exposure, specifically in the imprinted regions of the genome harboring ncRNAs and sequences interacting with regulatory proteins.
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The G-quadruplex sequences interacted with N-methylmesoporphyrin IX, obtaining an enhanced fluorescent signal.
It was designed to explore how viral fusion protein sequences interact with membranes.
To confirm that both of these sequences interact with E2F1, we conducted additional shift assays using longer probes corresponding to positions −166 to −104, including mutant probes in which one or both of the binding sites at −158 and −113 were altered (Fig. 4D).
These sequences interact with other proteins; for example, type I repeats bind to fibrin, and regions in type III repeats contain the RGD motive that binds integrins.
RNA immunoprecipitation (RIP-Seq) uses immunoprecipitation of an RNA-protein complex and subsequent reverse transcription and sequencing to identify which RNA sequences interact with a protein of interest [ 23].
Using ITC experiments, we quantified the associated affinities and found that the three Pdc1 HLM sequences interact with the Edc3 LSm domain between 150 μ m and lower mM range.
A growing body of research has shown specific amino acid sequences interact with the components of the exit tunnel, concomitantly influencing many aspects of translation such as the translation rate, ribosome stalling and even changes in mRNA frame maintanence.
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