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The ITS sequences in the present study were highly homologous (95 99%) with Zea ITS sequences reported by Buckler et al. [16], demonstrating that our sequences are accurate and reliable.
Thus, all P. glauca and P. abies sequences in the present study were annotated accordingly.
We noted an inconsistency of P. knowlesi sequences in the present study and the recently published genome report [ 44].
In addition, the Ca2+-binding EF-hand motif first identified in potato NDB [ 28] was found to be conserved among the NDB sequences in the present study.
This contrasts with 4.4% of white clover sequences in the present study, and 7% of EST-derived sequences in Barrett et al. [ 20].
Since the median length of the ChIP-chip sequences in the present study is 520 bases, we used test sequences of this length, centred on the known TRE.
Similar(50)
Three AIEC strains and three corresponding non-AIEC counterparts that shared identical PFGE patterns (Fig. S1), sequence types, phylogenetic origins and virulence genes (Table 1) with the AIEC strains were selected for genome sequencing in the present study.
All the genes sequenced in the present study except GH7 from R. miehei that was too short to produce any significant alignment and the genes found in NCBI (see Table 3) were aligned with MUSCLE before curating the alignments with Gblocks and building of phylogenetic trees with PhyML (Dereeper et al. 2008).
Our own analysis performed on comQXPA locus of B. licheniformis NCIMB 8874 as sequenced in the present study confirmed further evidences for this pattern of molecular evolution since the ComQ and ComX coding regions of strains WX-02 and NCIMB 8874 were found to share only 85% identity at the nucleotide level.
strain CI plasmids sequenced in the present study and those previously sequenced are outlined in Table 1.
When the C/D recombinant isolates sequenced in the present study were compared to those from other studies [16], [17], [23] using SimPlot and phylogenetic analyses, they were found to have similar recombination breakpoints (data not shown).
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