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The van't Hoff analysis of the temperature denaturation data showed that the DNA hybridization in our porous, flowthrough microarray is thermodynamically less favorable than the hybridization of the same sequences in solution.
We used high-throughput Monte Carlo simulations to simulate large ensembles of single-stranded RNA sequences in solution to validate the assumptions behind our diagrammatic scheme, examining the entropic costs for hairpin initiation and formation of many multiway junctions.
We have designed appropriately prepared solid supports consisting of poly methyl methacrylate) (PMMA) that provide enhanced performance levels for molecular beacons (MBs) that are used for recognizing and reporting on signature DNA sequences in solution.
The coupling of the single-stranded probe-DNA to VASWCNTS is achieved by coupling the amine-terminated probe-DNA in the presence of DCC in DMF solution with carboxylic acid-functionalized shortened SWCNTs, followed by hybridization of the immobilized probe to specific complementary DNA (cDNA) sequences in solution.
The covalently immobilised single-stranded DNA was able to selectively hybridise to its complementary DNA sequences in solution to form double-stranded DNA on the gold surface.
Current opinion considers that the poor solubility observed for many peptide sequences in solution reappears on the solid phase as difficult peptides and backbone protection acts in both cases by disrupting structure formation.
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A new DNA sample pre-treatment has been optimised to allow fast and simple detection of hybridisation reaction between a target sequence in solution and a probe immobilised on the sensing surface.
The Non-library Retroposons Enrichment Strategy has been conducted using plasmid Hmo41_It corresponding to the internal region of an individual SINE copy as probe to directly capture the HaeIII-fragmented genomic DNA containing the SINE sequence in solution.
In these arrays the probe sequences come in pairs: for each perfect match (PM) probe, which is exactly complementary to the transcript sequence in solution, there is a second probe with a single non-complementary nucleotide with respect to the specific target.
To expand the reaction scope and to study the feasibility of the one pot isomerization self-metathesis/cross-metathesis/reduction sequence in solution (Table 1), we decided to repeat the IMCR procedure for N-terminal-modified tetrapeptide 4 by application of the above-described reaction conditions.
In [8], Elabbasy et al. obtained the Fibonacci sequence in solutions of some special cases of the following difference equation x n + 1 = a x n − l x n − k b x n − p − c x n − q.
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