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The sequences in small capital indicate overlap sequence part and the sequences in all capital indicate primer sequence part used for Gibson assembly cloning.
Variable levels of rRNA sequences in small RNA libraries have been described previously [ 41, 42].
It is not clear whether these observations can be ascribed to chromosomal rearrangements or fragmentations of genomic sequences in small contigs.
If the goal is sequencing full-length genes, non-normalized libraries will yield a larger number of full-length sequences in small sequencing experiments compared to normalized libraries.
This information is exploited by miRNA-finding algorithms such as miRDeep2 for the identification of miRNA-like sequences in small RNA transcriptome data.
Multiplex ligation-dependant probe amplification technique is a high-throughput polymerase chain reaction (PCR -based method to determine the relative coPCR -basedof various DNA sequences in smethodamples of human DNA.
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The restriction sites of BamHI GGATCC, XhoI CTCGAG and SpeI ACTAGT (sequence in small letters underlined) were added to the end of the primer for conventional cloning.
Efforts, for example, also include genome-wide association studies (GWAS) to identify new candidate genes or whole genome sequencing in small, genetically homogeneous populations to find possible rare gene variants like copy number polymorphisms (CNPs) [19, 20].
However, deletions found in the sequencing in small scaffolds of Rut-C30 are located in regions with normal probe spacing in aCGH.
Recent investigations have suggested the feasibility of creating identification systems reliant on the analysis of sequence in small segments of DNA [ 31].
The primers used were pr128164N (5'-GGgctagcAccatggACACCGTCTACAACGGGAG-3'; the sequence in small letters represents the sequentially ordered NheI and NcoI sites; the NcoI recognition sequence provided an AUG initiation codon) and pr128164C (5'-TTgcggccgcGTTGAAGGAGGTAGGC-3'; the sequence in small letters represents a NotI site).
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