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The addition of more vertebrate OTX-class sequences in future analyses may help elucidate their relationship to the other OTX paralogues.
We recommend involving sequence and secondary structure information of chaetopeltidalean and chaetophoralean ITS2 sequences in future studies to find out if the monophyletic biflagellate DO-group could be further extended to a general monophyletic DO-group containing quadri- and biflagellate taxa.
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The identification of such extensive contamination of human sequence across databases and sequence types warrants caution among the sequencing community in future sequencing efforts, such as human re-sequencing.
The prevalence of the EDM1 sequence in future environmentally induced differential DNA methylation sites will need to be confirmed and further investigated.
Moreover, with the rapidly declining cost of sequencing, it would also be possible to increase the coverage and the number of birds for sequencing in future studies.
Therefore, bisulfite and miRNA sequencing in future experiments will be necessary to understand the relationship between epigenetic mechanism and transgenerational plasticity in germination behavior.
The database will be updated periodically based on the availability of the SNP/InDel datasets for genotypes which will be sequenced in future.
To extensively study the genes related the biosynthesis of secondary metabolites, we have plan to use other tissues of L.chinense for RNA sequencing in future.
Although the cost of traditional sequencing (over $1000/MB) has continued to decrease over the last decade, the lower cost of NG sequencing ($250/MB for GS20, $90/MB for FLX, and $5/MB for Solexa) will dramatically improve transcriptome sequencing in future research.
To test this hypothesis and examine the correlation between Sirt3 catalyzed histone deacrotonylation and gene expression genome-wide requires comprehensive profiling of global histone Kcr and gene expression regulated by Sirt3 using ChIP coupled to high throughput sequencing, in combination with RNA sequencing in future studies.
Despite this popular method has been validated by the statistician community [ 50], we recommend sequencing biological replicates rather than pooling them prior sequencing in future RNAseq analyses, as sequencing costs are now acceptable and the later approach allows a more robust identification of genes affected by variations across conditions.
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