Similarly, McGillivray and Husseini (1992) divided the Unayzah Formation in the Hawtah Hazmiyah fields into two informal sequences identified as Unayzah A and Unayzah B members.
The resulting dataset was comprised of 268 unique sequences identified as novel bovine miRNA and miRNA* (Supplemental Tables S3 S5).
It is a taxonomically rather consistent cluster, containing 27 sequences identified as H. olivaceus except for the GenBank sequences AF325641 ("H. bulliardii") and EU784360 ("H. citrinus").
CAMP [16] predicted dataset contained 1,153 sequences identified as antimicrobial based on the evidences of similarity or annotations in NCBI as 'antimicrobial regions' without exprerimental evidences.
Protein sequences identified as sHSPs were found in the UniProt [29] databank (release 15.9 of October 13 , 2009 that contains more than 10 million protein sequences.
All sequences identified as new miRNA precursors and the mature miRNA were submitted to the miRBase at the Sanger Centre (http://microrna.sanger.ac.uk/registry/).sanger.ac.uk/registry/
All of the nucleotide sequences identified as being differentially expressed (q<5%) from the agonist experiment were compared blast-2.2.19 blast-2.2.19e againstatheabovehrough Blast2GO [21].
Using this procedure on the UniProt databank, we found 4478 sequences identified as sHSPs, showing a very good coverage with the corresponding PROSITE and Pfam profiles.
Finally, the 46 P. falciparum putative serpentine receptor sequences identified by Inoue and colleagues [17] were analyzed using TMHMM 2.0, and the sequences with seven or eight transmembrane domains were selected and added to the sequences identified as explained above.
Reference ITS sequences from ascomycetous groups related to the Geoglossomycetes and ITS sequences identified as uncultured Pezizomycotina environmental samples were added to make a data set of 300 ITS sequences.
Third, we downloaded all ITS sequences identified as uncultured Pezizomycotina environmental samples from INSD, assuming some unidentified earth tongues might be found among them because of the position of earth tongues in the Pezizomycotina.
